Cellulases, a type of glycosyl hydrolase (GH), play a crucial role in cellulolysis by breaking down glycosidic bonds in cellulose. The cleavage of β-1,4-glycosidic bonds in cellulose through acid hydrolysis involves a proton donor, a nucleophile or base, and can result in inversion or retention of the anomeric configuration at the carbon-1 (C-1) of the […]